Analysis of Behavior and Trafficking of Dendritic Cells within the Brain during Toxoplasmic Encephalitis
Figure 2
CNS resident CD11c+ cells are efficient antigen presenting cells.
A) Representative z stacks (30 µm) of brain slices from CD11cYFP mice that received OT1GFP cells and were infected with Pru (left) or PruOVA (right) 3 weeks prior. B) Representative time series showing short term (blue circles) and sustained (red circles) interactions between OT1GFP and CD11cYFP cells. C) The average migration velocities of OT1GFP T cells and CD11cYFP obtained by manual tracking using Volocity. D) The frequency of OT1GFP cells interacting with CD11cYFP cells and the duration of these interactions is shown on the y-axis. The imaging sessions typically lasted between 12–15 minutes. E) CFSE dilution profile of naïve OT1 cells that were co-cultured (3 days) with CD11c+ cells purified from either spleen or brain (of chronically infected mice). F) The ability of the proliferated cells to produce the cytokines IFN-γ and IL-10, during re-stimulation (6 hours) in vitro in the presence of brefeldin-A, with/without antigenic peptide (SIINFEKL).