Plasmodium Protease ROM1 Is Important for Proper Formation of the Parasitophorous Vacuole
Figure 2
PyROM1 localizes with microneme markers and is not present at the parasite surface during sporozoite invasion.
(A) Immunofluorescence assay of blood stages shows partial co-localization of PyROM1-HA (R1HA) with the microneme protein pyMAEBL and the rhoptry neck marker RON4 in schizont merozoites. PyROM1-HA also partially overlaps the ER marker BiP in schizonts. (B) Immunofluorescence assay of pyROM1-HA (R1HA) salivary gland sporozoites reveals that pyROM1 partially co-localizes with the microneme marker PyMAEBL and with the eTRAMP protein PyUIS4 within diffuse intracellular granules throughout the length of the parasite body. Restricted localization that excludes the area surrounding the nucleus is observed between PyROM1-HA and the ER marker BiP. (C) Localization of pyROM1-HA during invasion into Hepa 1–6 cells. The extracellular portion of parasite stains with CSP. Intracellular localization of pyROM1-HA in a developing EEF at 4 hours post invasion into Hepa 1–6 cells has overlap with CSP.