Autocrine Regulation of Pulmonary Inflammation by Effector T-Cell Derived IL-10 during Infection with Respiratory Syncytial Virus
Figure 3
The blockade of effector T cell-derived-IL-10 enhances host morbidity without affecting viral clearance.
(A-D). BALB/c mice were infected with RSV and then were either injected with Rat IgG1 control mAb or α-IL-10R blocking mAb. (A) Mouse weight loss was monitored daily following infection. (B) Pulmonary function of naïve mice, Rat IgG1 mAb treated or α-IL-10R mAb treated d7 infected mice was monitored. P value was determined by unpaired two-tailed Student t test. * indicates P< = 0.05. (C) Airway virus titers from the indicated mice were determined by plaque assay. PBS, mice were infected with RSV and received PBS. P value was determined by unpaired two-tailed Student t test. n.s.(non-significant). (D) Lung RSV gene copies from the indicated mice were determined through quantitative RT-PCR. Flu, mice were infected with influenza A/PR8 virus. P value was determined by unpaired two-tailed Student t test. n.s.(non-significant). (E) Either WT or IL-10-/- naïve T cells were transferred into Rag1-/- mice which were then infected with RSV. Mouse weight loss was monitored daily after infection. A, B. Pooled data from three experiments are represented. C, D, E. Pooled data from two experiments are represented.