Effects of Interferon-α/β on HBV Replication Determined by Viral Load
Figure 4
Effects of HNF3 on HBV replication.
(A) Western-blot analysis for the expression of HNF3 isoforms. Liver nuclear extracts of HBV transgenic mice with (+) or without (−) poly(I∶C) injection were analyzed for HNF3α, HNF3β, HNF3γ, and lamin-β using their respective antibodies. (B) Effects of HNF3α, HNF3β and HNF3γ on HBV ENI/Xp activities in Huh7 cells. The ENI/Xp reporter construct pGL-3-1115-luc as well as the renilla luciferase reporter was co-transfected with the indicated amounts of the expression plasmid for HNF3α, HNF3β or HNF3γ into Huh7 cells. The control expression vector pRc/CMV was used to ensure that the total amount of DNA used in the transfection was the same among different experiments. The luciferase activities were determined as described in the Figure 3 legend. (C) Effects of HNF3γ on the ENI/Xp complex in mice. Nine-week old naïve mice were hydrodynamically injected with the ENI/Xp reporter construct, 8 µg 1.3mer HBV and the expression plasmid for either the control shRNA or the HNF3γ shRNA. Forty-two hours later, mice were further injected with poly(I∶C) or saline. Mice were sacrificed six hours later for the isolation of liver for the dual luciferase assay. Values represent means±S.D. of three independent experiments. The reporter activity in the absence of poly(I∶C) was arbitrarily defined as 100%. (D) Effects of HNF3γ on HBV replication in mice. Naïve mice were co-injected with 8 µg 1.3mer HBV and the expression plasmid for the control shRNA or HNF3γ shRNA, treated with saline or poly(I∶C) and sacrificed for the isolation of liver for HBV DNA and RNA analyses. Two mice were analyzed for each experiment for verification of the results.