A Permeable Cuticle Is Associated with the Release of Reactive Oxygen Species and Induction of Innate Immunity
Figure 4
Wounding in mutants of ABA biosynthesis.
Leaves were wounded and maintained for 1.5 h under high humidity in tightly covered well-watered trays (humid) or in uncovered trays at room conditions (dry) prior to ROS detection or infection with B. cinerea. W: wounded; Ctrl: unwounded control plants. (A) Densitometric quantification of ROS production in unwounded and wounded aba2, aba3 mutants and in WT plants. For ROS production (n = 4; ±SD), one representative image of the fluorescent leaf surface was placed above each histogram as a visual illustration. Different letters above each bar represent statistically significant differences (Dunn's test; P<0.05). (B) Effects of wounding on resistance to B. cinerea in aba2 and aba3 mutants and WT plants. After B. cinerea inoculation, all plants were kept under humid conditions (n = 32; ±SE); the experiment was repeated twice with similar results. Different letters above each bar represent statistically significant differences (Dunn's test; P<0.05).