In vivo Hypoxia and a Fungal Alcohol Dehydrogenase Influence the Pathogenesis of Invasive Pulmonary Aspergillosis
Figure 3
Normalized fold expression of pdc genes and alc genes in A. fumigatus under normoxic and hypoxic conditions.
(A) mRNA levels of all three pdc genes increase in hypoxia with pdcA being the most responsive. mRNA levels of the pdc genes show a reduction in hypoxia over time. (B) Of the three tested alc genes only mRNA from alcC is increased in response to hypoxia. Quantification of mRNA was achieved by quantitative real-time PCR. Values are normalized to β–tubulin and shown relative to normoxia. Results are the mean and standard deviation of three replicates.