HIV/SIV Infection Primes Monocytes and Dendritic Cells for Apoptosis
Figure 2
HIV-1 infected monocyte-derived macrophages and monocyte-derived DCs undergo apoptosis after stimulation.
(A) Monocyte-derived macrophages (MØ) or (B) monocyte-derived DCs were incubated with R5 HIV-1Bal (100 pg/ml of p24) or Mock control at day 1 after the initiation of APC differentiation and then stimulated at day 5 without or with LPS (10 ng/ml) and IFN-γ (103 U/ml) overnight. Apoptosis was assessed by flow cytometry using FITC-Annexin-V. One representative experiment out of three is shown. Percentages of apoptotic cells shown are means ± SEM (n = 3). Statistical significant differences, as compared to non stimulated cells, are indicated by an asterisk (p<0.05).