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Direct Visualization of Peptide/MHC Complexes at the Surface and in the Intracellular Compartments of Cells Infected In Vivo by Leishmania major

Figure 3

Flow cytometry analysis of LN cells.

Lymphocyte-depleted LN cells from 4 wk-infected BALB/c mice were analyzed by flow cytometry after staining with mAbs directed to CD11c, CD11b, Ly-6C, Ly-6G, F4/80, Siglec-F, CCR3, B220 and I-A/I-E. Data show representative FACS profiles after gating on the indicated populations. Numbers indicate mfi. (A) All cells. The percentage of DsRed+ cells in the gated population is indicated (left panel). The right panel shows the R1 and R2 gates that were used later in this study. (B) Eosinophils. (C) Dendritic cells. (D) Macrophages/monocytes. (E) Neutrophils.

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1001154.g003