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Direct Visualization of Peptide/MHC Complexes at the Surface and in the Intracellular Compartments of Cells Infected In Vivo by Leishmania major

Figure 2

Visualization of I-Ad/LACK complexes by immunogold electron microscopy.

BALB/c mice were injected into the hind footpads with 40 µg of LACK in CpG. LN cells were harvested 2 days later and enriched for CD11c+ cells using magnetic beads. Cells were frozen and ultrathin cryosections were analyzed by electron microscopy after immunogold labelling with 2C44 (upper panel), anti-I-A/I-E (middle panel) or control isotypic (lower panel) mAbs. Representative photographs are shown. Intracellular Compartments (IC) and Extracellular Compartments (EC) are indicated. Arrows point to dendrites.

Figure 2

doi: https://doi.org/10.1371/journal.ppat.1001154.g002