Direct Visualization of Peptide/MHC Complexes at the Surface and in the Intracellular Compartments of Cells Infected In Vivo by Leishmania major
Figure 2
Visualization of I-Ad/LACK complexes by immunogold electron microscopy.
BALB/c mice were injected into the hind footpads with 40 µg of LACK in CpG. LN cells were harvested 2 days later and enriched for CD11c+ cells using magnetic beads. Cells were frozen and ultrathin cryosections were analyzed by electron microscopy after immunogold labelling with 2C44 (upper panel), anti-I-A/I-E (middle panel) or control isotypic (lower panel) mAbs. Representative photographs are shown. Intracellular Compartments (IC) and Extracellular Compartments (EC) are indicated. Arrows point to dendrites.