A Crucial Role for Infected-Cell/Antibody Immune Complexes in the Enhancement of Endogenous Antiviral Immunity by Short Passive Immunotherapy
Figure 5
In vivo enhancement of CD8+ T-cell responses against FrCasE by ICs.
SpFr or SpFr-IC were administered i.v. to non-infected/non-treated mice. Four groups of mice (n = 3) where used: two of them were injected with either 2×106 or 2×105 FrCasE-infected splenocytes (SpFr and SpFr 1:10, respectively) in the absence of 667, and the other two were injected with the same amount of FrCasE-infected splenocytes complexed to 150 µg of 667 (SpFr-IC and SpFr 1:10-IC respectively). Nine days later, they were tested for the expansion of GagL-specific CD8+ T cells and for CTL activity in vivo as described in Figure 3. Non-infected/non-treated mice with no further treatment were used as controls (A). Proliferation of GagL-specific CD8+ cells. The statistical significance between groups was established using the Student's t test (*P = 0,0106; **P = 0,0049; ***P = 0,0071: ****P = 0,0352). (B) CTL activity against GagL-loaded splenocytes. A representative mouse of each group is presented.