A Differential Role for Macropinocytosis in Mediating Entry of the Two Forms of Vaccinia Virus into Dendritic Cells
Figure 8
Blocking components of the macropinocytosis pathway blocks viral gene transcription.
MDDCs were pre-treated with the LatA (10 µM), mβCD (2.5 mM), EGTA/AM (250 mM), rottlerin (10 µM) and wortmannin (200 nM) for 30 min prior to spinoculation with (A) MV-GFP (MOI 3) or (B) EV-GFP (MOI 1) at 4°C. Cells were washed and incubated at 37°C to allow virus entry for 2 h then lysed for analysis of immediate early viral transcripts E3L (shaded bars) and B2R (white bars) by qPCR. The expression of viral transcripts was normalised to expression of GAPDH and is expressed as a percentage of the untreated control from two independent donors with standard error bars.