Serological Profiling of a Candida albicans Protein Microarray Reveals Permanent Host-Pathogen Interplay and Stage-Specific Responses during Candidemia
Figure 3
Discrimination of convalescent candidemia patients from the study population.
(A) Two-way hierarchical cluster analyses of the 33 differentially expressed anti-C. albicans cell surface antibodies from early/mid convalescent candidemia sera. The heatmap is organized with antigens, in rows, and acute candidemia patients (n = 18), early and mid convalescent patients (n = 10) and negative control groups (hospital patients (n = 12) and healthy individuals (n = 50)) in columns. The colorized scale ranks the antigens with red being the strongest, bright green the weakest, and black in between. (B, C & D) Principal component analyses of serum anti-C. albicans cell surface IgG antibody expression profiles that discriminate between convalescent candidemia patients and each negative control group (hospital patients and health individuals). Each circle denotes the anti-C. albicans cell surface antibody profile of asingle serum specimen. Samples are color coded as the following acute candidemia patients (red), convalescent candidemia patients (brown), healthy individuals (green), and hospital patients (blue). (E) The graph shows the ROC curves generated using different sets of serodiagnostic antigens.