Regulation of Host Translational Machinery by African Swine Fever Virus
Figure 6
eIF4E and eIF4GI redistribution during ASFV time-infection steps.
A) Vero cells were seeded on glass coverslips and mock infected or infected with 5 pfu/cell of ASFV. Cells were then permeabilized and fixed at 4, 8, 16 and 24 hpi. eIF4E and eIF4GI were detected by indirect immunofluorescence and cell nuclei and ASFV factories were stained with To-Pro-3. Cells were visualized by confocal microscopy and the cell outline was defined by phase contrast microscopy. Images were obtained under restricted conditions and processed with Huygens 3.0 software. B) Vero cells were seeded on glass coverslips and mock infected or infected with 5 pfu/cell of ASFV. Cells were then permeabilized and fixed at 8, 10 and 18 hpi. eIF4GI and p72 were detected by indirect immunofluorescence and cell nuclei and ASFV factories were stained with To-Pro-3. Cells were visualized by confocal microscopy and the cell outline was defined by nomarski microscopy.