Deaminase-Independent Inhibition of Parvoviruses by the APOBEC3A Cytidine Deaminase
Figure 4
Alignment of APOBEC3 amino acid sequences for A3A with the C-terminus of A3B and A3G.
Residues exchanged in A3A are boxed and the mutant designation is indicated above. The PmlI site and stretches of variable sequence VS1 and VS2 switched in the chimeras are also indicated. The asterisks mark individual amino acid mutants, and the diamond indicates the start of A3G-CT (residues 197–384). Residue numbers are indicated on the right side. Predicted secondary structure of A3A is indicated below the alignment with α-helices in black and stranded β-sheets in grey. A3A secondary structure modeling was generated by Swiss-Model using the crystal structure of the C-terminal fragment of A3G (Protein Data Bank accession number 3E1A) as template [77].