Natural Transformation of Helicobacter pylori Involves the Integration of Short DNA Fragments Interrupted by Gaps of Variable Size
Figure 3
Alignment of methylation sites and regions containing multiple crossover endpoints in H. pylori DNA.
Each panel is a representation of ∼2500 bp flanking a StrR site in strain J99 or 26695. Each horizontal bar represents a region that contains at least two recombination endpoints: a, two endpoints within 100 bp; b, two endpoints within 24 bp; c, three endpoints within 68 bp; d, two endpoints within 70 bp; e, two endpoints within 200 bp; f, two endpoints within 15 bp; g, four endpoints within 100 bp. (A) Map of sites in chromosomal DNA from strain J99 recognized by active 26695 restriction endonucleases for which the corresponding methylase is absent in J99. HpyAII recognition sites (GAAGA) are designated by filled squares (■). HpyAV recognition sites (CCTTC) are designated by filled triangles (▲). (B) Map of sites in 26695 chromosomal DNA recognized by active J99 restriction endonucleases for which the corresponding methylase is absent in 26695. Hpy99IV recognition sites (CCNNGG) are designated by filled circles (●). Hpy99I recognition sites (CGWCG) are designated by filled diamonds (◆).