XIAP Regulates Cytosol-Specific Innate Immunity to Listeria Infection
Figure 7
XIAP enables synergistic cytokines responses to TLR and NLR ligands.
(A) IL-6 secretion from xiap+/y and xiap−/y activated BMDM treated with the indicated TLR ligands as measured by ELISA. Macrophages were activated overnight with 10 ng/ml LPS and 10 ng/ml interferon-γ. Cells were left untreated or were treated for 24 h with Pam3CSK4 (2 µg/ml), poly (I:C) (10 µg/ml), LPS (10 ng/ml), flagellin (10 ng/ml), imiquimod (5 µg/ml), or CpG DNA (1 µg/ml). Results are representative of at least 3 independent experiments (error bars represent SD). (B) IL-1β from the supernatants of xiap+/y and xiap−/y activated BMDM left uninfected or infected with wild-type or LLO− L. monocytogenes as measured by ELISA. Supernatants were collected at 8 hpi. Results are representative of 3 independent experiments (error bars represent SEM of cells from 6 animals). (C–E) ELISA of IL-1β (C), IL-6 (D), or TNF (E) secretions from xiap+/y and xiap−/y activated BMDM left untreated or treated for 8 h with MDP (10 µg/ml) and/or Pam3CSK4 (0.5 µg/ml). (F) QRTPCR analysis of IL-6 gene expression at 3 h in xiap+/y and xiap−/y activated BMDM treated with MDP (10 µg/ml) and/or Pam3CSK4 (0.5 µg/ml). The data shown are from the same experiment, but are represented on different graphs to show y values more accurately. Data are representative of 3 independent experiments with 3 mice each (error bars represent SD). *p≤0.05; **p≤0.005.