Legionella Eukaryotic-Like Type IV Substrates Interfere with Organelle Trafficking
Figure 6
The Predicted Transmembrane Domains of LegC2, LegC3, and LegC7 are Required for Their Localization in Yeast.
(A) Constructs of LegC2, LegC3 and LegC7 were designed to exclude the predicted transmembrane regions. The solid line drawn beneath each gene represents the amino acid sequence included in each final construct. The truncated constructs were translationally fused to gfp and transformed into NSY01 for expression. (B) Epifluorescence microscopy showing localization patterns. (C) Whole cell lysate immunoblot using a rabbit polyclonal antibody against GFP showing the truncated proteins.