Pathogenesis of Listeria-Infected Drosophila wntD Mutants Is Associated with Elevated Levels of the Novel Immunity Gene edin
Figure 4
Edin expression is partly regulated by the Imd pathway, and is correlated with increased S. typhimurium pathogenesis.
(A) Quantitative RT-PCR data for expression of edin in yw; wntD and yw control flies prior to and following infection with M. luteus. Expression is induced by infection with M. luteus, and expression is significantly elevated in wntD mutants following infection. (B) Quantitative RT-PCR data for expression of edin following infection with a mixture of gram-positive and gram-negative bacteria in various host genetic backgrounds. Induction is mitigated in imd10191 mutants, demonstrating input from the Imd pathway in controlling the expression of edin. Flies of the genotype spzrm7/spz2 express edin at higher levels than controls. Uninfected Tl10b/+ flies show mild induction of edin in the absence of infection (4.2 fold) (ND = this genotype was not assayed following infection). (C) Quantitative RT-PCR data for expression of edin in wild-type flies following infection with a wild-type strain of Salmonella typhimurium (SL1344) or a strain mutant for SPI1 and SPI2 (BJ66/P3F4). Values are relative to those in uninjected wild-type flies. Expression levels in all cases are normalized to Ribosomal protein 15a. Error bars indicate s.e.m. Asterisks indicate significance by student t-test: * = p<0.05, ** = p<0.01.