Transcriptional Regulation of Multi-Drug Tolerance and Antibiotic-Induced Responses by the Histone-Like Protein Lsr2 in M. tuberculosis
Figure 9
Preferential Binding of Lsr2 to Circular DNA: Agarose Gel Shift of Linearized versus Circular DNA
Four hundred nanograms of either linear or circular pCV125 or pG21898–12 (pCV125 containing the PiniBAC sequence) were analyzed in the presence of different amounts of Lsr2 (600 ng of Lsr2 in lanes 2, 7, 12, and 17; 400 ng in lanes 3, 8, 13, and 18; 200 ng in lanes 4, 9, 14, and 19; and 100 ng in lanes 5, 10, 15, and 20). No Lsr2 was added to the control lanes 1, 6, 11, and 16. After 120 min of incubation at room temperature, the samples were analyzed on a 1% agarose gel.