An in vitro workflow of neuron-laden agarose-laminin hydrogel for studying small molecule-induced amyloidogenic condition
Fig 1
A neuron-laden agarose-laminin hydrogel.
A) A workflow diagram for cell-laden agarose-laminin scaffold preparation B) The morphology of 3D culture in two different platforms; scaffold (top) and scaffold-free (bottom) culture at day 2, 4, and 6 post-seeding. At day 6 of cell culture under scaffold-free platform, SH-SY5Y cells were aggregated and began to attach on the plastic surface (red arrow). Scale bar represents 50 μm. C) The expression of mature neural marker, TUJ1 of scaffold and scaffold-free 3D cultures at day 7. Bar graph represents the mean ± SE (n = 3). n.s: not statistically different.