Uncovering in vivo biochemical patterns from time-series metabolic dynamics
Fig 3
Comparing ethanol profiles under different growth conditions through FPCA.
Two different carbon sources were compared: natural abundance glucose (3 experiments) and uniformly 13C-labeled pyruvate (2 experiments). The glucose experiments were all done at a high density (10 mg/63 μL), and the pyruvate experiments were done at low (6mg /63 μL, solid lines) and high (10 mg/63 μL, dashed lines) densities. The ethanol features from the glucose experiments are shown in red. The 13C-labeled ethanol produced in the 13C-pyruvate experiments is shown in brown. The unlabeled ethanol produced in the 13C-pyruvate experiments is shown in green. A: FPCA score plot indicates the overall patterns of ethanol in each of these experiments. Each point represents one ridge in one sample. The small, grey points correspond to all the other ridges detected in these experiments. The X (Y) axis represents scores for PC 1 (2). B: Time trajectory (hours) of the highlighted features from A. Each curve was centered and scaled for A and B. Similar plots for other compounds can be found in S3 Fig.