Quantification of human plasma metalloproteins in multiple sclerosis, ischemic stroke and healthy controls reveals an association of haptoglobin-hemoglobin complexes with age
Fig 5
Zn-specific chromatograms obtained after the analysis of plasma from MS participants, AIS participants and healthy controls.
Column: Superdex 200 Increase 10/300 GL (30 x 1.0 cm I.D., 8 μm particle size), Temperature: 22°C, Mobile phase: 150 mM PBS buffer (pH 7.4), Flow rate: 0.75 mL/min, Injection volume: 500 μL. ICP-AES detection at 213.86 nm (Zn). Retention times of molecular weight markers are depicted on top. 750 μL of plasma was diluted with 250 μL of PBS buffer and 500 μL of the obtained solution were injected on the SEC column.