Predicting drug targets by homology modelling of Pseudomonas aeruginosa proteins of unknown function
Fig 1
A large portion of the genomes of P. aeruginosa PA01 and WHO priority pathogens consist of GUFs.
A) The majority of P. aeruginosa GUFs are found only in Pseudomonas species (specific), while some of them are conserved in other organisms (nonspecific). The analysis was performed using the PGD version 20.2 download on 5.11.2020. B) Conservation of P. aeruginosa genes of unknown function in WHO priority pathogens. P. aeruginosa PUFs were used as a query for a BLAST search on NCBI to find protein homologues (>35% sequence identity, >75% sequence coverage) in the following thirteen WHO priority pathogens. Taxonomic identifiers of organisms are listed in S2 Table. C) Pathogen-specific genes, essential genes, virulence factor genes and genes with human homologues among GUFs. Numbers in brackets indicate the number of genes in each group. A number of P. aeruginosa GUFs in overlapping sections is indicated as the number of specific genes/number of nonspecific genes. Numbers on lines indicate mutual GUFs among two linked groups.