Physiologically relevant aspirin concentrations trigger immunostimulatory cytokine production by human leukocytes
Fig 6
Exogenous addition of PGE2 antagonizes most of the immunostimulatory effects of ASA.
PGE2 (5 ng/ml) was added to citrate-anticoagulated blood in the presence or absence of 0.2 mg/ml ASA before stimulation with TLR-ligands including (A) Pam3CsK4 (B) HKLM (C) LPS (D) Flagellin and (E) ssRNA40. Stimulated cells incubated without ASA and PGE2 (vehicle) set as 100%. Data represent mean ± SD of three experiments performed in triplicate.