Maltohexaose-indocyanine green (MH-ICG) for near infrared imaging of endocarditis
Fig 1
a) Maltohexaose-azide was conjugated with ICG-alkyne through a copper-catalyzed click reaction. b) Analytical HPLC confirmed the purity of MH-ICG. Purified MH-ICG was injected onto an analytical Xbridge C18 5 μm 4.6 x 150 mm column. (Flow rate: 1 ml/min. Solvent system: MeCN/H2O + 0.1% TFA, 0–20 min: 40% MeCN, 20–40 min: gradient to 100% MeCN.) Product eluted at 27.2 min. c) Fluorescence spectrum of MH-ICG. The absorbance and fluorescence intensities are indicated as solid and broken lines, respectively. The emission spectrum was generated with an excitation wavelength of 760 nm.