Genetic deletion of connexin 37 causes polyuria and polydipsia
Fig 5
Renal abundance and phosphorylation of AQP2 is reduced under vehicle and impaired in Cx37-/- compared to WT mice in response to V2R activation.
Sixty minutes after vehicle application Cx37-/- mice had lower abundance of (A) total and (B) phosphorylated serine 256 AQP2 compared with WT mice. Systemic administration of dDAVP following suppression of endogenous arginine-vasopressin overnight (see Materials and methods for details) increased the abundance of (A) total and (B) phosphorylated serine 256 AQP2 in both genotypes; however, the effect was attenuated in Cx37-/- mice. No differences in the abundance of V2R or AQP3 were observed between genotypes or treatments. Data are expressed as mean ± SEM; n = 5 each genotype and condition. Data were analyzed by paired and unpaired Student’s t-test, *P<0.05 versus WT same condition, #P<0.05 versus vehicle same genotype.