Red elemental selenium nanoparticles mediated substantial variations in growth, tissue differentiation, metabolism, gene transcription, epigenetic cytosine DNA methylation, and callogenesis in bittermelon (Momordica charantia); an in vitro experiment
Fig 6
MSAP-based variation in DNA methylation patterns of bitter melon in response to the incorporation of nSe at 0 (control) and 30 mgL-1 into the MS culture medium.
(A) A representative gel image exhibiting polymorphic fragments in control and nSe-treated group. (H) DNA digested under EcoRI/HpaII conditions. (M) DNA digested under EcoRI/MspI conditions. (B) PCoA plot showing variation in methylation-susceptible loci (MSL). (C) graph displaying differences in DNA methylation; HPA+ and MSP+ (1, 1) showing unmethylated; HPA+ and MSP- (1, 0) referring to hemimethylated; HPA- and MSP+ (0, 1) internal cytosine methylation; HPA- and MSP- (0, 0) exhibiting full methylation.