Structure-function investigation of 3-methylaspartate ammonia lyase reveals substrate molecular determinants for the deamination reaction
Fig 3
MAL activity on β-lysine monitored using 1H NMR.
(a), Spectrum corresponding to 60 mM 6-AHEA in reaction buffer. (b), Spectrum corresponding to β-lysine incubated with CaMAL at 30°C for 4 d in reaction buffer. (c), Spectrum corresponding to the control reaction in which β-lysine was incubated in reaction buffer for 4 d at 30°C.