Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-self-recognition system
Fig 8
Real time qPCR validation of Argopecten ventricosus juvenile transcriptome data for selected genes.
(A) Relative expression normalised to the abundance of sodium/potassium-transporting ATPase subunit alpha-like gene in mantle tissue and correlation between Log2FC transcriptome data and RT-qPCR data in mantle; (B) Relative expression normalised to the abundance of cAMP-dependent protein kinase catalytic subunit-like isoform X3 gene in gill tissue and correlation between Log2FC transcriptome data and qPCR data in gill. IAP: Baculoviral IAP repeat-containing protein 3-like, HSP90: Heat shock protein 90, ERIS: Endoplasmic reticulum interferon stimulator, SRC: Src substrate cortactin-like isoform X1, HEXA: Beta-hexosaminidase, CAT: Catalase, TRAF3: TNF receptor-associated factor 3, FILA: Filamin-A-like isoform X3, TLR2: Toll-like receptor 2, TLR3: Toll-like receptor 3. T1: Vibrio parahaemolyticus and Vibrio alginolyticus lysate 1D; T2: V. parahaemolyticus and V. alginolyticus lysate 7C; T3: Silicea terra® and Phosphoric acid® (Similia®, CDMS, MX) 7C; T4: Vidatox® (Labiofam®, Habana, Cuba) 31C, and control treatment C1: hydro-alcoholic solution (1%).