High-yield production of recombinant platelet factor 4 by harnessing and honing the gram-negative bacterial secretory apparatus
Fig 10
A three-dimensional representation of the synergistic effects on the rPF4 secretion of supplementation with various concentrations of glycine, Triton X-100, and IPTG.
(A) In the absence of Triton X-100, the best secretion level is achieved by moving toward lower IPTG concentrations near 0.1 mM. In addition, tweaking glycine concentration does not seem to result in any significant change in the protein secretion level. (B) When the cocktail medium is supplemented with 0.25% Triton X-100, the optimum secretion point is achieved by moving toward zero glycine concentration and 0.1 mM IPTG. (C) At 0.5% Triton X-100 concentration, the optimum secretion level is brought about at zero glycine concentration and 0.05–0.1 mM IPTG.