High-glucose diets induce mitochondrial dysfunction in Caenorhabditis elegans
Fig 1
Glucose affects the morphology of mitochondria and endoplasmic reticulum of germ cells.
(A-E). Electron micrographs of germ cells of worms that were exposed from L1 to L4 larval stages to glucose 20, 40, 80 or 100 mM that show damaged mitochondria. (A) In control worms, mitochondrial cristae membranes are fine and dim (faint) (small arrows). (B) A widening of the endoplasmic reticulum cisternae was observed at glucose 20 mM, (C) larger at 40 mM and then (D) diminished at 80 mM. Mitochondrial external membrane showed extensive rupture, and a higher amount of endoplasmic reticulum was observed in worms grown at glucose 100 mM (E). G, Golgi cisternae; V: vacuoles. 50000X magnification; scale bar represents 500 nm.