Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis
Fig 6
Ubiquitin colocalizes with MVEs in cells expressing STUB1-DN mutants.
ARPE-19 cells were transduced using lentiviral particles containing vectors for the expression of either STUB1K30A or STUB1H260Q. Control cells were transduced with empty vector. A) Immunofluorescence using confocal microscopy with antibodies against myc, ubiquitin and the RhoB-PE dye for MVE labeling shows an increase in puncta positive for ubiquitin, STUB1-DN mutants and RhoB-PE. B) Quantification of size and number of vesicles labelled with RhoB-PE dye shows an increase in the frequency of larger vesicles in the STUB1-DN mutants.