Two novel and correlated CF-causing insertions in the (TG)mTn tract of the CFTR gene
Table 3
Primers used for cDNA amplification and sequencing.
Pairs F4-R4 and F4-R5 were used for RT-PCR. Primer F4 was used for cDNA forward sequencing; primers R4 and R5 were used for cDNA reverse sequencing. The position of the nucleotide at 5’-end of each primer is indicated in both legacy and HGVS name. The annealing temperatures used (Ta) and the length of amplicons are indicated.