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Vinculin and metavinculin exhibit distinct effects on focal adhesion properties, cell migration, and mechanotransduction

Fig 2

MVcn-expressing cells have larger but fewer FAs compared to Vcn-expressing cells.

(A) Western blot shows the expression level of either Vcn or MVcn in Vcn null MEF background. Both expression levels are equivalent to endogenous Vcn expression in WT MEFs. (B) Fluorescent images of WT MEF, Vcn-null parent MEF cell line, exogenous Vcn-expressing, and MVcn-expressing Vcn-null MEFs stained for paxillin and showing expression of either fluorescently-tagged Vcn or MVcn. Scale bar = 10 μm. FA number (C) and overall FA area per cell (D) were quantified. Graphs represent data pooled from 4 independent experiments (n ≥ 100 cells; ***, p<0.001).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0221962.g002