Liposomal delivery of ferritin heavy chain 1 (FTH1) siRNA in patient xenograft derived glioblastoma initiating cells suggests different sensitivities to radiation and distinct survival mechanisms
Fig 2
Liposomal delivery of siRNA efficiently downregulates FTH1 expression in GICs.
(A) Western blot analysis of FTH1 expression for T3691 cells transfected with siRNA: liposome ratio of 2μg:4μl. (B) Densitometric quantification of T3691 cells in (A) showed significant reduction in FTH1 expression relative to control siRNA treated cells at 24h (57%,p<0.01), 48h (86%, p<0.001) and 72h (79%,p<0.001).(C) Western blot analysis of FTH1 expression in T387 cells transfected at ratios of 2μg:4μl. (D) Densitometric analysis of T387 cells in (C) showed comparatively poor knockdown at 24h (9%, p<0.05) and 48h (49%, p<0.001) with the maximum knockdown achieved at 72h post transfection (63%, p<0.001). (E) Western blot of FTH1 expression in T387 cells transfected at 2μg:8μl. (F) Densitometric analysis of (E) showed that changing siRNA: liposomes ratio to 2:8 in T387 cells improved knockdown significantly at 24h (45%, p<0.001), 48h (66%, p<0.001) and 72h (44%, p<0.01). Expression levels were normalized to endogenous actin levels and then to expression of FTH1 in liposome treated controls. Percentage values are relative to control siRNA from three independent experiments.