Biphasic in vitro maturation with C-type natriuretic peptide enhances the developmental competence of juvenile-goat oocytes
Fig 1
Effect of pre-IVM with CNP and estradiol on the maintenance of meiotic arrest in juvenile-goat oocytes.
(A) Germinal vesicle (GV) rate of oocytes cultured for 6 h and 8 h with different CNP concentrations. A total of 47–48 oocytes were stained per condition (4 replicates). (B) GV rate of oocytes cultured for 6 h and 8 h with different CNP concentrations plus estradiol. A total of 46–50 oocytes were assessed per condition (4 replicates). Each bar represents mean + SEM. Different superscript letters (a-c) in each column indicate statistically significant differences (P<0.05). (C) Relative gene expression of natriuretic peptide receptor (NPR2) in COCs after 6 h of pre-IVM with CNP and E2. Five samples were tested per group (10 COCs per sample). Each bar represents relative quantification (RQ), and error bars show RQ max and RQ min. Superscript symbols indicate statistical differences relative to control 0 h: (*) P<0.001; (**) P<0.0001.