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Epitope mapping of the protease resistant products of RT-QuIC does not allow the discrimination of sCJD subtypes

Fig 5

10%BH seeded RT-QuIC reaction kinetics and PK resistant RT-QuIC reaction products.

A) Averaged RT-QuIC traces per individual seed are displayed along with the number of replicates that reached the fluorescence threshold for positivity. (B) RT-QuIC reaction products from reactions displayed in (A) were digested with PK (50 ug/mL) and probed by WB with mAb SAF70 1:1000. Samples are grouped according to sCJD strain of prions as defined in [8] and sub-grouped according to sCJD subtype as defined in [6]. Lane MM: Molecular weight marked; lane rec: 10 ng of FLHa-rPrP; Lane PBS: unseeded reactions; Lane Mix: RT-QuIC untreated reaction mixture.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0218509.g005