Inflammatory response of mesenchymal stromal cells after in vivo exposure with selected trauma-related factors and polytrauma serum
Fig 3
(A) Analysis of cytokines in serum from healthy volunteers (control; n = 5), in AB-serum (n = 1) and in serum from polytrauma (PT) patients (n = 5). Mean and SD are shown. Cytokine levels in serum from PT patients were compared to cytokine levels in serum from healthy volunteers. Statistical significance was assessed via student's t-test (with correction for multiple comparisons), * P < 0.05. IL6: interleukin 6; IL10: interleukin 10; CXCL1: C-X-C motif chemokine ligand 1; CCL2: C-C motif chemokine ligand 2. (B) Effect of serum from PT patients on proliferation of MSC. Three MSC preparations and five individual sera from PT patients were used (randomized combination). Serum was collected from PT patients 0/4/12/24/48/120/240 h after admission to hospital. MSC cell count was determined by fluorescence intensity and compared to cell growth in Alpha-MEM without (negative control) or with supplement (positive control).