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ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells

Fig 1

Sequence of the anti-IFNα intrabody αIFNα-ib and soluble scFv αIFNα-ab.

A, Scheme of αIFNα-ib and αIFNα-ab. VH and VL were PCR amplified from the cDNA of the complete IgG antibody 4EA1 and a scFv constructed with either a myc tag and ER retention signal or a His-tag. B, Shown are the coding (upper lane) and amino acid sequence (lower lane) including the exons and introns of the signal peptide, the (Gly4Ser)3 linker, the myc epitope tag and the ER retention signal. The original antibody has a histidine at position 10 of VH (marked with an asterisk). The scFv αIFNα-ab sequence contains a His6-tag instead of the ER retention signal. Complementary determining regions (CDRs) are shown as blue arrows.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0215062.g001