Distribution of Brucella field strains isolated from livestock, wildlife populations, and humans in Italy from 2007 to 2015
Fig 1
Brucella AMOS-PCR profiles: In order to identify Brucella species and biovars, for each strain, 4 PCR have been performed: AMOS multiplex PCR, Omp 2a PCR, Omp 2b PCR and Omp 31 PCR.
In this image, this four PCR have been grouped to obtain a good glance and easily identify the Brucella profile. Lane 1 and 22 Marker molecular weights; Lane 2–5: Amos (2)-Omp2a (3)-Omp2b (4)-Omp31 (5) B. abortus biovar 3,5,6,9; Lane 6–9: Amos (6)-Omp2a (7)-Omp2b (8)-Omp31 (9) B. suis biovar 2,3,4,5; Lane 10–13: Amos (10)-Omp2a (11)-Omp2b (12)-Omp31 (13) B. melitensis biovar 1,2,3; Lane 14–17: Amos (14)-Omp2a (15)-Omp2b (16)-Omp31 (17) B. abortus biovar 1,2,4; Lane 18–21: Amos (18)-Omp2a (19)-Omp2b (20)-Omp31 (21) B. ovis.