Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust
Fig 2
PCR products of PLUG markers in wheat-D. villosum alien introgression lines.
(a)-(e) PCR amplification generated by markers TNAC1248, TNAC1294, TNAC1301, TNAC1277 and TNAC1267, respectively; M, Trans2k plus DNA markers (Trangene, Beijing, China); 1, common wheat CS “Chinese Spring”; 2, D. villosum, 3–4, CS-D. villosum 1V -2V additional lines; 5, CD-3; 6–9, CS-D. villosum 4V -7V additional lines. The asterisks indicated the D. villosum-specific bands and the arrows showed the 3D-specific band which was absent in CD-3.