Can the antral follicular count modulate the gene expression of bovine oviducts in Aberdeen Angus and Nelore heifers?

The number of visible ovarian antral follicles (antral follicle count—AFC) is repeatable in bovine individuals, but highly variable between animals, and with differences between Bos taurus and Bos indicus breeds. Several studies have tried to determine the correlation between AFC and increased fertility in cattle. While the impacts of AFC on embryo production, hormonal levels, and pregnancy rates have been described, the molecular effects of AFC on bovine oviducts have not yet been investigated. Here, the aim was to investigate the impact of breeds, such as Aberdeen Angus and Nelore heifer with high or low AFC, on abundance of transcripts and protein related to oviductal transport, sperm reservoir formation, monospermy control, and gamete interaction in the oviducts. In summary, the ovulation side was the major factor that affected transcript abundance on bovine oviducts. However, a discreet effect among AFC and cattle breeds was also observed. Based on this, we concluded and reinforced here that differential microenvironments between ipsilateral and contralateral oviducts have a major effect on modulating the transcripts related to oviductal transport, sperm reservoir formation, monospermy control, and gamete interaction. However, we cannot exclude that there is minimal effect of AFC or breed on regulation of some genes (such as AGTR1, ACE1, FUCA1, and VEGFA) in bovine oviducts.


Introduction
Several studies have investigated the relationship between the ovarian antral follicle count (AFC) and bovine fertility [1,2]. There is evidence that AFC is highly variable among different animals, but it is constant within the same animal during their reproductive life [3][4][5][6]. This allows for distinguishing between animals with a high (HFC) and low follicle count (LFC). A high number of follicles per wave is directly associated with an increased efficiency in PLOS

Animal selection
This study was conducted on a farm located in Ribeirão do Sul (São Paulo, Brazil; latitude −22˚47 0 03 00 ; longitude −49˚56 0 01 00 ; altitude 479 m). Heifer selection and AFC group classification were previously described by Loureiro et al. [32]. Briefly, using ultrasound examination (US, Mindray, 5-10 MHz, China), the total number of follicles was determined in 100 Aberdeen Angus and 100 Nelore heifers in a random day of estrus cycle. All selected heifers were cycling (with CL presence) and had no follicles greater than 5 mm. Then, these heifers were synchronized with two doses of PGF2α 11 days apart. Four days after the second PGF2α (approximately 24 hours after follicle recruitment), another US evaluation was performed to confirm the total number of follicles in each heifer. Considering the mean of AFC ± standard deviation (SD) in each breed, the heifers were classified into two groups: LFC (animals with a total number of follicles below the mean-SD) and HFC (animals with a total number of follicles above the mean + SD). For oviductal analysis, 16 heifers with an age of 24 months, a body condition score of 4 (0, emaciated; 5, obese), 345 kilograms (mean of Nelore heifers) and 330 kilograms (mean of Aberdeen Angus heifers) were used in the present study. The study analyzed eight Nelore heifers (n = 4/each AFC group) and eight Aberdeen Angus heifers (n = 4/each AFC group; Fig 1). The numbers of follicles were 15 ± 1 (LFC) and 53 ± 3 (HFC) in Nelore heifers, and 9 ± 2 (LFC) and 33 ± 2 (HFC) in Aberdeen Angus heifers (values presented as mean ± SD). All animals were studied simultaneously, at the same place and time while maintained on a pasture (Brachiaria brizantha), with ad libitum access to water. They were fed 2 kg of Cynodon spp. hay and 4 kg of concentrate (16% crude protein and 70% total digestible nutrients) per animal, per day for a total of 90 days. were classified according to their ovarian AFC: low follicular count (LFC, n = 4) and high follicular (HFC, n = 4). All 16 animals were slaughtered one day after ovulation, and both oviducts were obtained. The ipsilateral and contralateral to ovulation side from each oviduct, including the infundibulum, ampulla, and isthmus segments, were submitted to relative gene expression by real time RT-PCR and protein quantification by western blotting.

Sample collection
To synchronize the estrous cycle, the heifers were given two doses of prostaglandin F 2 a spaced over 11 days, and then the ovaries were evaluated by US every 12 hours until ovulation. The heifers were slaughtered in a commercial abattoir 24 hours after ovulation. One or two animals from each experimental group were slaughtered in each of the three independent sessions.
Blood samples were collected at the day of the slaughter, centrifuged (10 minutes at 900 x g), and the plasmatic antimullerian hormone (AMH) concentration was measured to confirm the phenotypes of the experimental animals [10]. Plasmatic AMH concentration in Nelore was higher (102.3 ± 6.4 pg/ml; p <0.001) when compared with Aberdeen Angus (78 ± 5.1 pg/ml) heifers. Moreover, the AMH concentration was higher (p <0.001) in heifers with HFC when compared to the LFC from Nelore (127 ± 8.8 and 84 ± 7.5 pg/ml) and Aberdeen Angus (82 ± 6 and 72 ± 6.9 pg/ml).
The reproductive tracts were then transported to the laboratory (approximately 2 hours of transportation) in saline solution (0.9%) at 4˚C. Ipsilateral and contralateral oviducts of the ovulation side of each animal were isolated, and the surrounding connective tissues were trimmed. The oviduct length was measured by a ruler, and then the oviducts were divided by segment: infundibulum, ampulla, and isthmus (the transition regions were discarded). Two fragments of each segment were collected and stored in −80˚C until gene and protein analysis (Fig 1).

Sample preparation
Tissue samples (20 mg) were homogenized separately in CK28-R tubes (2 mL, with ceramics beads) by a Precellys 1 homogenizer (Bertin Technologies 1 , Montigny le Bretonneux, France) after adding 500 μL lysis buffer, as follows: three cycles of 50 seconds at 6500 rpm with 15 second intervals. Total RNA and total protein were extracted using Illustra TriplePrep Kit (GE Healthcare, Buckinghamshire, UK), according to the manufacturer's instructions.
Total RNA (1.2 μg) from each sample was incubated with DNAse I (Invitrogen 1 , CA, USA) and then reverse transcribed with a High Capacity cDNA kit (Applied Biosystems™, Carlsbad, CA), according to the manufacturer's instructions. Relative RT-qPCR analysis was performed using TaqMan 1 Low Density Array cards according to the manufacturer's instructions (TLDA, Applied Biosystems™, Carlsbad, CA). The TLDA cards (384 wells) were designed with 24 genes in duplicate to analyze eight samples in each TLDA card (Table 1). Briefly, the TLDA card is a ready to use system, with selected TaqMan Gene Expression Assays pre-loaded into each of the 384 reactions (250 nM, final concentration 1 μL reaction volume). Individual samples were diluted with water to a final volume of 50 μL (total RNA load: 600 ng) and mixed with 50 μL TaqMan 1 Universal PCR Master Mix (2X). Each mix (100 μL) was loaded in one of the eight channels of the TLDA card. The cards were sealed, spun, and submitted to standard PCR conditions: 50˚C for 2 minutes, followed by 95˚C for 1 minute, then 40 cycles of 95˚C for 15 seconds, and 60˚C for 1 minute in the ViiA7 PCR machine (Thermo Fisher Scientific). The intrassay variation CV for all PCR analysis was 15% of the cycle-threshold value.

Western blotting
Total protein quantification was performed by the Bradford method in 96 well plates, and the samples were analyzed by a spectrophotometer at 595 nm. Aliquots (70 μg of protein) were treated with a buffer solution (Laemmli sample buffer BIO-RAD) and beta-mercaptoethanol at 100˚C for 5 minutes. The proteins were then separated by SDS-PAGE and transferred to a nitrocellulose membrane. Nonspecific binding of proteins was blocked by incubating the membrane in 5% skim milk-TBST buffer for 1 hour at room temperature. The membranes were incubated with primary antibody FUCA-1 (rabbit polyclonal ab-98310, 1:1000, Abcam Inc., Cambridge, MA) or GAPDH (rabbit polyclonal sc47724, 1:1000, Santa Cruz Biotechnology VR, Inc., Dallas, TX) in 5% skim milk in TBST at 4˚C overnight.
After washing four times in TBST, membranes were incubated with specific HRP secondary antibody (IgG goat-anti rabbit, ab97051, 1:20,000, Abcam Inc.) in 5% skim milk in TBST for 2 hours at room temperature. After washing four times in TBST, immunoreactive components were visualized by chemiluminescence (ELC Select TM Western Blotting Detection Reagent, GE Healthcare 1 , UK). Protein expression was tested in a subset of samples (total of 8 animals), as a pre-analysis of transcript genes indicated minimal effect concerning breed and AFC. The protein abundance was determined by semi-quantitative assays through band densitometry using Image J software (version 1.33u, National Institutes of Health, USA), normalized by GAPDH density. The integrated optical density (IOD) of the band was used as the unit of measure; mean and standard error of the mean (SEM) of IODs were compared among the groups and submitted to statistical tests.

Statistical analysis
For all analyses, except for total oviduct length measurement, each oviduct segment was evaluated separately. Oviduct lengths were transformed to logarithmic values for a normal distribution and then tested by ANOVA (the cows were divided into two groups: Nelore and Aberdeen Angus, as there was no influence of AFC for this analysis). For gene expression analysis, each target gene was normalized using the geometric mean of two reference genes (18S and PPIA) and one calibration sample by 2(-ΔΔCt); [43]. Gene expression values were analyzed to determine the effect of triple interactions (breed vs. AFC vs. ovulation side; n = 4 animals/group), double interactions (breed vs. AFC, breed vs. ovulation side, and AFC vs. ovulation side; n = 4 animals/group), and main effects (breed, AFC, and ovulation side; n = 8 animals/group). Analysis of the ovulation side (ipsilateral and contralateral) considered them as dependent samples, since they come from the same animal. The responses were estimated by fitting linear mixed models after transforming the responses to a logarithmic scale. Multiple comparisons were performed by using the Bonferroni correction of p-values.
The total protein quantification by Bradford assay was analyzed to determine the effect of breed, AFC, and ovulation site. The responses were estimated by fitting linear mixed models after transforming the responses to a logarithmic scale. Multiple comparisons were performed by using the Bonferroni correction of p-values. Each oviductal segment was analyzed separately. For protein quantification by western blotting, each oviductal segment was analyzed separately, and only the difference in ovulation side (ipsilateral and contralateral) was evaluated for protein abundance. Relative abundance of FUCA1/GAPDH was estimated using Image-J, and then the data were transformed to the logarithmic for a normal distribution and tested with a Student's t-test.
All analysis was performed using Proc mixed, SAS version 9.3 (SAS, 2010-2015). The differences were considered significant when p 0.05, and data are represented by mean ± SEM.

Gene expression
All target genes were detected in the three bovine oviductal segments, except for the LHCGR transcript, which was not detected in half of the samples and impaired the analysis of its expression. Furthermore, not all genes were affected by differences in heifer breeds, AFC, or ovulation side (Table 2). In general, gene expression analysis was not affected by triple interaction (breed vs. AFC vs. ovulation side) and the isolated AFC characteristic. The most constitutive effect on the transcriptional profile of oviducts is caused by the ovulation side. Regarding isolated breed effects, only mRNA abundance of AGTR1 was higher in Aberdeen Angus when compared to Nelore in the ampulla and isthmus segments (Fig 2B and 2C).
In the infundibulum, higher mRNA abundance of OVGP1 and FUCA1 was present in the ipsilateral when compared to the contralateral oviduct from Aberdeen Angus. A similar effect was not present in Nelore heifers, and there was no significant difference between breeds ( Fig  3A and 3B). Moreover, the relative abundance of ACE changed based on the side of the LFC heifers (higher levels in the ipsilateral compared to contralateral), but not in the HFC (Fig 3C). When only comparing the ovulation side in the infundibulum, a higher mRNA abundance of HSPA5, FUCA2, ANXA2, VEGFA, and PTGER4 was present in the ipsilateral oviduct when compared to the contralateral (Fig 4).
In the ampulla, mRNA abundance of FUCA1 was higher in the ipsilateral than in the contralateral oviducts in LFC heifers, but this effect was not detected in the HFC heifers, and there was no significant difference between AFC groups in the same ovulation side (Fig 5). Regarding ovulation side, mRNA abundance of five genes was affected; OVGP1, HSPA5, ANXA4, PTGS2, and END1 mRNA were higher in ipsilateral ampulla when compared with the contralateral (Fig 6).
In the isthmus, a lower number of genes was affected. The mRNA abundance of VEGFA was lower in ipsilateral when compared to the contralateral isthmus from LFC heifers, but no difference was detected in the HFC animals, and there was no difference between different AFC groups in the same ovulation side (Fig 7). Regarding the difference in the ovulation side, mRNA abundance of FUCA2 and KDR was lower in the ipsilateral isthmus when compared to the contralateral (Fig 8).

Protein abundance
The cattle breed, AFC, and ovulation side had no effect on the total protein levels in the infundibulum and isthmus. However, in the ampulla, the AFC had a significant effect (p < 0.01) with greater levels in the LFC (8.99 ± 0.68 μg/μL) than the HFC (5.70 ± 0.84 μg/μL). When comparing the ipsilateral and the contralateral oviducts (Fig 9), the relative abundance of FUCA1 in the infundibulum, ampulla, and isthmus was similar.

Discussion
To our knowledge, for the first time, the impact in variation of the antral follicle count (AFC) on oviductal gene expression was evaluated in two cattle breeds used for beef production. Genes reported as potentially responsible for oviductal transport, sperm reservoir formation, monospermy control, and gamete interaction presented a minimal difference in the oviducts comparing animals with high or low AFC, independent of the cattle breed. However, a clear interaction between AFC and the oviduct side indicates that HFC animals have similar gene abundance in both oviducts, while the abundance of some genes in LFC animals is very different when the ipsilateral and contralateral are compared. After the phenotypic classification of heifers based on variation in AFC (low vs. high), several studies have tried to determine the correlation of AFC increased fertility in cattle. It is clear that there are benefits for HFC animals when compared to LFC regarding reproductive biotechnology, including ovarian superovulation and in vitro embryo production, as the total number of structures (oocytes and embryos) is higher in HFC animals [46]. However, the efficiency of embryo production is controversial, some studies observed a similar result between animals with different AFC [1,47], better in HFC animals [2], or better in LFC animals [46]. Other studies have demonstrated normal sizes of corpus luteum and endometrial thickness between AFC groups [4], while other studies have showed poor endometrial development [4] and lower protein content in the uterus of LFC animals [11]. These findings suggest AFC variation is not a clear factor in bovine fertility modulation.
In this present work, we demonstrated an interaction between AFC and the ovulation side regarding the mRNA abundance of some genes. The HFC animals have no difference between ovulation sides; however, in LFC animals, some factor may regulate differential abundance of ACE (in the infundibulum), FUCA1 (in the ampulla), and VEGFA (in the isthmus) between ipsi-and contralateral oviducts. Moreover, LFC animals presented a higher total protein concentration in the ampulla when compared to HFC animals. Taken together, these findings might not solve the discussion about the relationship of AFC and bovine fertility, but we suggest that there is no detrimental effect in the oviduct of animals with LFC. There are some physiological differences between breeds, e.g., estrous cycle length, size of pre-ovulatory follicle, and steroid hormone concentration (estradiol and progesterone [48][49][50][51][52]). In the present study, the total length of Aberdeen Angus (Bos taurus taurus) oviducts were longer than those of Nelore (Bos taurus indicus) heifers. We theorized that this difference in length suggests that gametes and embryos from Aberdeen Angus have a longer course during transport in the oviduct compared to Nelore heifers, and perhaps some compensatory mechanism is necessary to guarantee that the gametes and embryos are transported on time. Indeed, mRNA abundance of AGTR1 in the ampulla and isthmus from the oviducts of Aberdeen Angus was higher than that of Nelore heifers. Angiotensin II (AGTII) participates in

Differences in transcripts levels in the infundibulum (ovulation side/breed and ovulation side/AFC).
Relative mRNA abundance (mean ± SEM) of OVGP1 (A) and FUCA1 (B) in ipsilateral and contralateral oviducts from Aberdeen Angus (n = 8 animals) and Nelore (n = 8 animals) heifers were normalized by reference genes using 2 (-ΔΔCt). The analyses were performed by comparing different ovulation sides in the same breed and the same ovulation side in different breeds, as well as relative mRNA abundance of ACE (C) in ipsilateral and contralateral oviducts from LFC (n = 8 animals) and HFC (n = 8 animals) heifers. The analyses were performed by comparing different ovulation sides in the same AFC groups, and the same ovulation sides in different AFC groups. Different uppercase letters ( A,B ) indicate significant differences (p 0.05) between ovulation sides in the same breed/AFC group (horizontal analysis). Different lowercase letters ( a,b ) indicate significant difference (p 0.05) between breed/AFC of same ovulation side (vertical analysis). reproductive physiology [53] by interacting with at least two receptor subtypes-AGTR1 and AGTR2 [54]. The AGTR1 receptor mediates effects of AGTII in the oviduct to stimulate smooth muscle contraction [34] and CBF [25]. Therefore, the upregulation of AGTR1, in the present study, may cause a stimulatory effect on oviductal CBF and faster transport in the oviducts of Aberdeen Angus heifers, making AGTII a potential factor in the compensatory mechanism of this breed.
Despite this, we observed that the major modulations in genes related to oviductal functions were associated with ovulation side. Fertilization and embryo initial development are regulated by oviductal fluid (OF) production, and the identification of specific proteins from OF is important in the understanding of physiology and for the application of this knowledge to reproduction biotechnology. In the present study, the ovulation side modulated the levels of OVGP1 (infundibulum from Angus heifers), OVGP1 (ampulla from all animals), and HSPA5 (infundibulum and ampulla from all animals) transcripts; they were higher in the ipsilateral compared to the contralateral oviduct, suggesting an increased functional activity of these proteins in ipsilateral segments. Oviductal glycoprotein 1 (OVGP1) is a protein present in the oviduct related to zona pellucida (ZP) solubility modifications, and consequently enhances sperm penetration resistance [31, 42] and leads to monospermy [42,55]. Heat shock 70 kDa protein 5 (HSPA5, previously known as Glucose-Regulated Protein, 78 kDa, GRP78) interacts with sperm, improving their viability, acrosomal integrity, and sperm movement [39, 56,57]. HSPA5 also modulates sperm-ZP interactions [58] and possibly participates in ZP hardening mechanisms, regulating monospermy levels [31]. Therefore, current data suggests that upregulation of OVGP1 and HSPA5 in ipsilateral oviducts could be involved in the prevention of polyspermy by modulating ZP hardening, even before fertilization [31] (Fig 10).
FUCA is an acidic glycosidase that catalyzes the hydrolytic degradation of fucose [38]. It is present in the reproductive system [59] and involved in different roles mediated by OF. In cattle, there are two types of fucosidases, FUCA1 (α-L-1-fucosidase) and FUCA2 (α-L-2-fucosidase), and the difference in function of each protein in the oviduct is not yet understood. In the present study, both FUCA1 and FUCA2 were detected in all oviduct segments. In the isthmus, sperm enters the oviduct and binds to ciliary epithelial cells-more specifically to fucose residues-for reservoir formation [29]. FUCA present in the OF regulates sperm release from the isthmic reservoir, controlling the number of sperm reaching the fertilization site [29], which is increased after ovulation [60]. Surprisingly, in the present study, FUCA2 abundance was lower in the ipsilateral isthmus compared to the contralateral side, one day after ovulation. It is possible that sperm interaction is more involved in isthmus regulation. Several studies have showed the importance of sperm in isthmus modulation, and confirmed a cross-talk between gamete and oviductal cells [61][62][63][64][65][66]. The absence of sperm in the experimental setup of the present study might be responsible for the downregulation of genes in the ipsilateral isthmus region (Fig 10).
Previous studies showed the participation of fucosidase during fertilization and embryo development. Pre-incubation of oocytes with FUCA decreased the number of sperm bound to ZP [67], while the inhibition of FUCA activity reduced sperm penetration during bovine in Expression of the target gene was normalized by references genes using 2(-ΔΔCt), and relative abundance is presented as mean ± SEM. The analyses were performed by comparing different ovulation sides in the same AFC groups, and the same ovulation side in different AFC groups. Different uppercase letters ( A,B ) indicate significant difference (p 0.05) between ovulation sides in the same AFC group (horizontal analysis). Different lowercase letters ( a,b ) indicate significant difference (p 0.05) between AFC groups on the same ovulation side (vertical analysis). https://doi.org/10.1371/journal.pone.0202017.g005 Effect of antral follicular count on bovine oviduct vitro fertilization [68,69], and in turn, the oocyte was unable to pass the 2-pronuclear stage [69]. In the present study, higher levels of FUCA1 (Angus heifers) and FUCA2 (all animals) in the ipsilateral infundibulum linked to an upregulation of FUCA1 in the ipsilateral ampulla of LFC heifers, and suggests a positive regulation of fucosidase by ovulation; perhaps to guarantee fertilization and embryo development. On the other hand, quantification of FUCA1 protein did not differ between ipsilateral and contralateral segments. One possibility is that the total amount of FUCA measured was from the entire oviduct, and not only from the secreted protein present in the OF.
After identification of fucose as a sperm binding site [29], Ignotz and collaborators [28] identified the location of the receptors, demonstrating the participation of four annexins in bovine sperm binding, ANXA1, ANXA2, ANXA4, and ANXA5, which are present in the cilia of the oviductal isthmus epithelium. The present study also showed the presence of all annexins in the ampulla [28], which indicates the continuous sperm binding to ampulla epithelial cells [70][71][72]. Moreover, ANXA4 shows ion and water regulation movement across human endometrium cells [73]. Higher levels of ANXA2 and ANXA4 in the ipsilateral infundibulum and ampulla, respectively, could be involved in sperm binding and/or controlling ion and water movement regulation across oviductal cells. The upregulation of ion and water movement in the ipsilateral oviduct by high levels of ANXA4 could explain the results of Kolle et al. [16]; they observed a thicker wall of the ipsilateral oviduct, which is more edematous and transparent than the contralateral oviduct (Fig 10).
Another important function of the oviduct is the transportation of gametes to the fertilization site and embryos to the uterus [74]. This is possible due to the presence of cilia beats and the contraction of the oviductal smooth muscle [75,76]. Local production of prostaglandins (PTG), endothelin-1 (END-1), ANGII, and vascular endothelial growth factor (VEGF) is involved in the control of oviduct transport [21,34,35,77,78]. The association of prostaglandin F2α (PGF2α) and prostaglandin E 2 (PGE 2 ) results in a rhythmic control of contracting Effect of antral follicular count on bovine oviduct and relaxing smooth muscle cells, respectively [79]. Higher levels of PTGs and END-1 are present in the ipsilateral oviduct side from the developing dominant follicle and in the ovulation side [80], which increases amplitude and frequency of contractile activity in the oviduct [20,37]. PGE 2 receptors are involved in ciliary beating control, and the presence of PTGER2 and PTGER4 on epithelial cell surface was shown to support the stimulatory effect of PGE 2 on CBF in hamster oviducts [81] and human uterine tube [82]. Additionally, functional studies showed that ANGII had a stimulatory action on CBF in human uterine tube [25]. In the present study, ipsilateral infundibulum presented higher levels of VEGF and PTGER4 in all animals, and ACE in LFC heifers, when compared to the contralateral side. These results corroborate the stimulatory effect of an LH and E 2 combination (hormonal profile of peri-ovulatory stage) on mRNA expression of VEGF [35]. VEGF expression could upregulate the biosynthesis of PGTs, END-1, and ACE in the infundibulum to stimulate CBF, as it does not possess smooth muscle [83], and the CBF has a positive impact on ovum pick-up and transport to the fertilization site. Moreover, there was an upregulation in the ipsilateral ampulla of PTGS2 and END1 levels, suggesting a positive influence on transportation in the ampulla (Fig 10).
In contrast to the infundibulum and ampulla, gene expression in the isthmus showed an opposite behavior. VEGF levels in LFC heifers and KDR levels (VEGF receptor) in all animals were lower in the ipsilateral isthmus, when compared to the contralateral side. Wijayagunawardane et al.
[35] described a negative feedback mechanism of VEGF on its own system. Combined with LH and E 2 , VEGF blocks the stimulatory effect of LH and E 2 , and Effect of antral follicular count on bovine oviduct downregulates the oviductal VEGF system after ovulation. This is to suppress oviductal contraction to safely and slowly transport the embryo to the uterus at the required time [35]. Regulation of the VEGF system in the isthmus is possibly due to preparing this segment for receiving the presumable zygote and guaranteeing the correct speed of transport to the uterus (Fig 10).
Therefore, the answer to the initial question of this study (if the gene expressions of Aberdeen Angus and Nelore heifer oviducts are modulated by the antral follicular count) is partially minimal in our experimental context. It appears that the key factor in modulating the genes reported as potentially responsible for oviductal transport, sperm reservoir formation, monospermy control, and gamete interaction, one day after ovulation, is the differential microenvironment between ipsilateral and contralateral sides. Paracrine and autocrine factors produced by the preovulatory follicle or ovulation products might be responsible for modifying the oviductal microenvironment in preparation of the environment for fertilization. These factors would control the gene expression in the infundibulum and ampulla to guarantee correct The ovulation side was the major factor that modulates the molecular profiles of bovine oviducts. There is a possibility of modification in the ipsilateral oviduct microenvironment to control gamete transportation, polyspermy gamete interaction, and embryo development even without the occurrence of mating, sperm presence, or fertilization. These oviductal functions could be controlled by ovulation products. 1. Upregulation of VEGF, PGE2, and ANGII systems in the infundibulum increase CBF, resulting in efficient ovum pick-up and oocyte transportation to the fertilization site. 2. Upregulation of PTGs and END1 systems in the ampulla possibly raises oviductal transport by stimulatory effects on smooth muscle contraction or CBF. 3. Downregulation of the VEGF system in the isthmus, caused by negative feedback, guarantees slow and safe transport of the embryo to the uterus. 4. Upregulation of annexin transcripts in the ampulla possibly results in an increase of sperm-bound sites, thereby controlling sperm release to oocyte fertilization. 5. Upregulation of OVGP1, HSPA5, and FUCA levels from the infundibulum to the ampulla may increase the activity of these factors on the oocytes before fertilization, participating in ZP hardening, control of sperm bound to ZP, and embryo development. We observed a difference between ipsi-and contralateral oviducts concerning the abundance of ACE (in the infundibulum), FUCA (in the ampulla), and VEGFA (in the isthmus) from low-AFC cows, but they were similar in both sides of the oviducts in high-AFC cows. The modulation by the AFC is still inconclusive, and more experiments should be performed to confirm if this subtle effect of AFC on oviduct functions can impact animal reproduction. AFC: antral follicle count, CBF: ciliary beat frequency, ZP: Zona Pellucida; CL: Corpus Luteum; (−) downregulated factor, (+) upregulated factor. Black large arrows represent the OVGP1, HSPA5, and FUCA coming from the infundibulum and ampulla. https://doi.org/10.1371/journal.pone.0202017.g010 Effect of antral follicular count on bovine oviduct gamete and embryo transport, polyspermy control, and gamete interaction. Therefore, the differential fertility capacity of different cattle breeds and AFCs might present a minimal impact on oviductal gene expression and might not be the principal component involved in bovine oviductal function. But our results support further studies to maximize understanding of the impacts of the cattle breeds and the AFC in the bovine oviduct physiology and function.