Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression
Fig 5
Effects of FcγRIIIA polymorphisms on the activity of NK cells.
FcγRIIIA polymorphisms were determined by flow cytometric analysis and divided into the VV/VF (n = 8) and FF groups (n = 4). Differences in CD137 expression on the surface of NK cells in both groups were measured with flow cytometry at the end of Tmab-induced ADCC against Panc-1 (5A, left panel) and Capan-1 (5A, right panel). Levels of IFN-γ (5B, upper panels) and TNF-α (5B, lower panels) released from NK cells at the end of the ADCC assay were measured using ELISA.