Absence of IL-10 production by human PBMCs co-cultivated with human cells expressing or secreting retroviral immunosuppressive domains
Fig 3
Characterisation of the concentrated TM proteins of HIV-1, PERV, and MuLV.
(A) Quantification of the TM proteins using an ELISA-based method. Ni(2+) chelate-coated 96 well plates are used to quantify 50 μl of batches which are 40 x concentrates of secreted TM proteins. To quantify the concentration of collected and secreted TM proteins a standard curve of highly purified polyhistidine tagged gB protein from HHV-6 was used (S2 Fig) (B) Results of LAL testing using 50 μl concentrated supernatants, samples were measured in duplicates.