Proteomics analysis identified peroxiredoxin 2 involved in early-phase left ventricular impairment in hamsters with cardiomyopathy
Fig 3
Representative 2D-DIGE image and images of spots with significant alterations in LV lysates.
(A) Proteins (40 μg each) were labeled with Cy3 and Cy5 dyes, mixed and subjected to 2D-DIGE analysis. Cy3- and Cy5- images are illustrated using red and green pseudocolors, respectively. Images were analyzed using DeCyder software. IPG strips (pI 3–11) were used for IEF, and 12.5% SDS-PAGE for the second dimension. (B) The 2-DE images of 4 spots showed significant alterations in the left ventricles of both TO-2 and Bio 14.6 hamsters, compared with F1B control hamsters.