Saccharomyces cerevisiae as a platform for assessing sphingolipid lipid kinase inhibitors

Successful medicinal chemistry campaigns to discover and optimize sphingosine kinase inhibitors require a robust assay for screening chemical libraries and for determining rank order potencies. Existing assays for these enzymes are laborious, expensive and/or low throughput. The toxicity of excessive levels of phosphorylated sphingoid bases for the budding yeast, Saccharomyces cerevisiae, affords an assay wherein inhibitors added to the culture media rescue growth in a dose-dependent fashion. Herein, we describe our adaptation of a simple, inexpensive, and high throughput assay for assessing inhibitors of sphingosine kinase types 1 and 2 as well as ceramide kinase and for testing enzymatic activity of sphingosine kinase type 2 mutants. The assay was validated using recombinant enzymes and generally agrees with the rank order of potencies of existing inhibitors.


Introduction
The sphingolipid kinases (sphingosine kinase (SphK) and ceramide kinase (CerK)) catalyze the transfer of phosphate from ATP to the primary hydroxyl of dihydrosphingosine (sphinganine), phytosphingosine, sphingosine, (SphK) or dihdyroceramides, N-acyl phytosphingosines and ceramides (CerK). Most eukaryotes have two sphingosine kinase genes while only a single ceramide kinase gene has been identified but some eukaryotes, e.g. Saccharomyces cerevisiae, lack detectable ceramide kinase activity. There have been recurrent suggestions that these enzymes might be therapeutic targets, most prominently for oncology indications. However, these suggestions are often based on genetic-based studies or on the use of sub-optimal inhibitors. Thus there is a need for improved chemical tools, the discovery of which is dependent in part on facile biochemical assays.
A widely used assay for sphingolipid kinases involves incubating the enzyme with sphingosine and γ-[ 32 P]ATP (or γ-[ 33 P]ATP), extracting non-polar compounds into an organic solvent mixture and detecting the radioactive product by autoradiography after normal phase thin layer chromatography [1]. In our experience this assay is both reliable and sensitive and it can be used with either recombinant enzymes or cell/tissue lysates. However, the assay is cumbersome, time consuming and too low throughput to support a medicinal chemistry campaign. Alternative assays have been developed to obviate the use of radioactive substrate and/ or to eliminate the organic solvent extraction step. Such assays use fluorescently labeled [2] or biotinylated [3] substrates or capture the radiolabeled S1P on cation exchange paper [4], on streptadivin-coated paper [3] or on the walls of a 96 well plate [5]. Further, mass spectrometry based methods have been described [6], and a kit for detection of the ADP product is available (e.g. Promega's ADP-Glo™ Kinase Assay). All of these assays are more expensive and/or lower throughput relative to the yeast-based assay discussed herein. Given our need for a higher throughput, inexpensive assay for sphingosine kinases to assess new chemical entities as potential inhibitors, our attention was drawn to a recent report by Kashem et al. [7] describing the use of the budding yeast, Saccharomyces cerevisiae, as a platform to screen for human SphK1 inhibitors. S. cerevisiae is widely used in drug discovery because of the ease of culturing and low cost, although the organism can be problematic in that some chemotypes do not readily accumulate. Our particular assay takes advantage of the toxicity of excessive levels of phosphorylated LCBs (Long Chain Bases, i.e. sphinganine, phytosphingosine) to S. cerevisiae [8][9][10][11]. In wild type yeast, phospho-LCBs are degraded by two catabolic enzymes-a phosphatase (encoded by the LCB3 gene) and a lyase (encoded by DPL1). Mutant yeast strains null for these gene products have high levels of phospho-LCBs and grow poorly [8,9] or not at all [11]. However, if the genes encoding the phospho-LCB generating kinases (LCB4, LCB5) are deleted also, normal growth is restored to the quadruple null mutant strain [8].
Forced expression of human SphK1 in the quadruple mutant (LCB3Δ DPL1Δ LCB4Δ LCB5Δ) strain results in severely retarded growth [7]. Importantly, Kashem et al. [7] demonstrated that growth of such a yeast strain can be rescued in a dose dependent fashion by adding an SphK1 inhibitor to the growth media. This result suggests that the products of the enzyme (phospho-LCBs), rather than forced expression of the human protein, are responsible for the toxicity. Herein, we report the extension of the findings of Kashem et al. to additional sphingolipid kinases, disclose uses of the assay beyond screening for SphK1 inhibitors and discuss the assay's limitations and advantages based on our experience.
The plasmids pGAL-HsSPHK1, pGAL-HsSPHK2, pGAL-MmSphk1, pGAL-MmSphk1L277M, pGAL-MmSphk2, pGAL-HsCERK and pGAL-SK2_MOD used in this study were constructed by sub-cloning DNA encoding the indicated translational open reading frames into the pYES2-FLAG-URA expression vector (provided by Dr. Cungui Mao). The encoded proteins all have an amino terminal FLAG epitope tag (DYKDDDDK) and their expression is under the control of the GAL1,10 promoter. Excepting human and mouse SphK2, the DNA sequences are synthetic (from GeneWiz LLC (South Plainfield, NJ)) and were optimized for expression in S. cerevisiae. The Genbank accession number designations are provided in Table 1.
Yeast harboring plasmids were selected and maintained on synthetic complete media lacking uracil (SC-URA) with 2% glucose as the fermentable carbon source. After overnight growth at 30˚C in this media, the cultures were diluted 1:100 into SC-URA media supplemented with 2% galactose and various concentrations of test inhibitor. After a further 24-48 hours incubation at 30˚C, the extent of growth was assessed by measuring absorbance at 600 nm. Assays were conducted using either 3-5 mL of media in 16x150 mm glass tubes or 0.1 mL of media in Nunc U-bottom 96 well plates (VWR catalog # 62409-116) sealed with a gas-permeable membrane (Breathe Easy Plate Seals, VWR catalog # 102097-158). Tubes were incubated in a rotating drum while plates were agitated using a plate mixer. A 600 for 96-well plate cultures was determined using a Molecular Devices SpectraMax M5 plate reader. Test compounds were dissolved in DMSO at 40-50 mM and diluted into water containing 0.1% fatty acid free bovine serum albumin. In all cases, the DMSO concentration in the assay was less than 2% (v/v).

Construction of human SphK2 deletion mutants
To facilitate construction of deletion mutants of human SphK2, we altered the underlying DNA sequence to introduce a set of restriction enzyme sites that, on digestion and re-ligation, maintained the translational open reading frame (confirmed in each case by DNA sequencing). While some of the nucleotide changes were translationally silent, the altered human SphK2 (termed 'SK2_MOD') DNA encoded five amino acid changes (Glu71Asp, Gly138Ser, Glu146Asp, Glu437Gly, Gly623Ala).

Determination of protein expression
The expression of plasmid-encoded sphingolipid kinases was determined using the protocol of Zhang et al. [12]. Yeast cells expressing deletion mutants of N-terminal FLAG-tagged human SphK2 were grown overnight on SC-URA media with 2% glucose. The cultures were diluted 1:100 into SC-URA media supplemented with 2% galactose in presence of an SphK2 inhibitor (0.5 μM SLM6031434) for 24-48 hours. Cells from 1 mL culture fluid were collected by centrifugation, re-suspended in 2 mL of 2M lithium acetate and incubated on ice for 5 minutes. This procedure was repeated and cells were suspended in 2 mL of 0.4M NaOH and incubated on ice for 5 minutes, re-centrifuged and the pellets were re-suspended in 0.25 mL of 1X Laemmli buffer and heated to 95˚C for 5 minutes. After clarification by centrifugation, the supernatant fluid (10-15 μL) was loaded on 4-20% polyacrylamide gels and the resolved proteins were transferred onto a nitrocellulose membrane. Membranes were blocked with 5% (w/v) non-fat dried skimmed milk powder in TBS (Tris-buffered saline, pH 7.4) containing 0.1% Tween 20 for 1 hour at room temperature. After rinsing, membranes were incubated with monoclonal anti-FLAG M2-peroxidase antibody (Sigma-Aldrich #A8592) for an additional hour. After washing three times in TBS, the blot was developed by chemiluminesence using a commercial kit (PerkinElmer Western Lightning).

Quantification of yeast sphingolipids by liquid chromatography / mass spectrometry (LCMS)
Cell pellets (approximately 5 million cells) were mixed with 2 mL of a 3:1 methanol/chloroform mixture and transferred to a capped glass vial. To this suspension was added 10 μL of internal standard solution containing 1 μM d7S1P (deuterated sphingosine 1-phosphate) (Avanti Polar Lipids). The mixture was homogenized in a bath sonicator for 10 min and incubated at 48˚C for 16 h. The mixture was then cooled to room temperature and mixed with 200 μL of 1 M KOH in methanol. The samples were again sonicated and incubated at 37˚C for 2 h. After this time, the samples were neutralized through the addition of 20 μL of glacial acetic acid and transferred to 2 mL microcentrifuge tubes. Samples were then centrifuged at 10,000 x g for 10 min at 4˚C. The pellets were discarded, the supernatant fluid was collected in a separate glass vial and the solvent was evaporated under a stream of nitrogen gas. Immediately before LCMS analysis, the material was dissolved in 300 μL of methanol, clarified by centrifuged at 12,000 x g for 12 min at 4˚C, and analyzed by LCMS. Analyses were performed using a triple quadrupole mass spectrometer (Sciex 4000 Q-Trap) coupled to a Shimadzu LC-20AD liquid chromatography system. A binary solvent gradient with a flow rate of 1 mL/min was used to separate sphingolipids by reverse-phase chromatography using a Supelco Discovery C18 column (50 mm × 2.1 mm, 5 μm bead size). Mobile phase A consisted of water/methanol/formic acid (79.9:20:0.1, by volume), whereas mobile phase B was methanol/formic acid (99.9:0.1, v/v). The run started with 100% solvent A for 0.5 min after which solvent B was increased linearly to 100% solvent B over 5.1 min and held at 100% solvent B for 4.3 min. The column was finally re-equilibrated to 100% solvent A for 1 min. Sphingolipids were detected using MRM (multiple reaction monitoring) methods as follows: d7S1P (387. 4

Results
In adopting this assay platform, we first attempted to replicate the results of Kashem et al. [7] using both human SphK1 and SphK2. We were successful, specifically, we observed that forced expression (by growth in media wherein the sole fermentable carbon source is galactose) of either human isoform in the quadruple null strain (CBY169) inhibited growth, while forced expression of a sphingosine kinase was not severely growth inhibitory so long as either LCBphosphate catabolic gene (DPL1 or LCB3) was intact (Fig 1). We extended this observation to another sphingolipid kinase, human ceramide kinase (HsCerK), the expression of which was growth inhibitory to our standard laboratory strain, JS1256 ('WT').
All four inhibitors restored growth in galactose media in a dose-dependent fashion ( Fig  3A). The EC 50 value determined for 9ab (0.9 μM) is similar to the K I value (1.4-1.7 μM) that we determined previously for this compound using recombinant human SphK1 [5]. The other three compounds featured in Fig 2A restored full growth in a dose dependent manner and the rank order potency of the compounds was the same as determined at recombinant human SphK1 (SLP7111228 > VPC96091 > SLC5111312) [4,16,17]. Further, we tested SLC5111312 (a dual SphK1/2 inhibitor [17]) and SLM6031434 at human SphK2 and found both compounds likewise rescued growth (Fig 3B) with the same rank order potency as established with K I values (SLM6031434 > SLC5111312).
We also tested the selective human SphK1 inhibitor, PF-543, on human SphK1 expressing yeast on the CBY169 background, but this compound failed to rescue growth at concentrations up to 30 μM (Fig 3C). Such a failure might be due to either cytotoxicity or failure of the compound to accumulate (penetration − (extrusion + metabolism)) sufficiently in the yeast cells.
In an attempt to increase accumulation of PF543 in the yeast, we modified CBY169 to generate a new strain (KYA1) wherein PDR5 was deleted. PDR5p is an ABC (ATP Binding Cassette) transporter that confers resistance to a variety of xenobiotics [20]. This gene is commonly deleted in S. cerevisiae to decrease extrusion of test compounds. As shown in Fig 3C, PF-543 restored full growth of the KYA1 strain expressing human SphK1 in a dose dependent manner, albeit with a EC 50 value (5.7 μM) that is about three log orders higher than the reported K I value of this compound [12]. Unlike PF-543, the potency of SLM6031434 was the same regardless of PDR5p status (CBY169 vs. KYA1 strains) (Fig 3C). We also tested SKI-II (non-selective) and ABC294640 (SphK2 selective), but those inhibitors failed to rescue growth of SphK1 or SphK2-expressing yeast on either the CBY169 or KYA1 backgrounds. We found SKI-II to be toxic when present at concentrations above 3 μM, which obviates the use of the assay for assessing this low potency (K I 12-30 μM [5]) compound. ABC294640, although not cytotoxic, failed to rescue growth of KYA1 yeast expressing human SphK2 at concentrations up to 100 μM. We next asked whether ceramide kinase's (CerK) growth suppression phenotype (see Fig 1) could be reversed by adding a CerK inhibitor to the media. To our knowledge, neither ceramide kinase activity nor the predicted products of the enzyme (dihydroceramide 1-phosphate, phytoceramide 1-phosphate) have been observed in S. cerevisiae. When we forced expression of human CerK in our standard laboratory strain (JS1256) by culturing pGAL-Hs-CERK harboring yeast in galactose media, the cultures failed to grow but growth was restored in a dose dependent fashion by addition of the CerK inhibitor, NVP-231 [19] to the culture media ( Fig 3D). This result suggests that human CerK expression is toxic to yeast due to dihydroceramide 1-P and/or phytoceramide 1-P accumulation or, perhaps, to reduction of the enzyme's substrates (dihydroceramide and phytoceramide). We note that a problem intrinsic to ceramide biochemistry, i.e. the difficulty in manipulating naturally occurring ceramides, which are water-insoluble, is circumvented with the yeast assay.
The data presented in Fig 3 are from single point assays, that is, only one determination was made at each inhibitor concentration. To estimate the variability of the assay in our hands, we performed 4 independent assays wherein the SphK2 selective inhibitor, SLM6031434, was used to rescue growth of human SphK2-expressing CBY169 strain yeast and compared the EC 50 values so determined. These values were (in nM) 78, 127, 142, 144 (mean = 123, 95% confidence interval 92-153 nM). A second estimation of variability was achieved by performing the assay in 96 well plates whereby the SphK1 inhibitor, VPC96091, was assayed in triplicate. The variation among the data points is shown in Fig 4. We note that the yeast cultures in the plates achieve nearly the same density as in individual 16 x 150 mm tubes, but this is not revealed in the maximum absorbance measurements from plate readings because of the limited linear range of the plate reader (culture media from tube assays was diluted 10-fold prior to determining A 600 ).
To confirm that forced expression of a sphingosine kinase resulted in high levels of phosphorylated LCBs, we cultured yeast harboring either a pGAL-HsSPHK1 or a pGAL-HsSPHK2 Yeast were cultured for 6 hours in the indicated media; inhibitors were present at 300 nM. Sphingolipids in cell pellets were analyzed by LCMS (see Methods for details).
Encouraged by our success with S. cerevisiae as a sphingolipid kinase inhibitor assessment tool, we considered further applications of the yeast system for investigating sphingolipid kinase biology. The yeast system enables rapid interrogation of mutant enzymes for activity (i. e. inhibition of growth on galactose) and the potency of inhibitors at mutant enzymes (i.e. rescue of growth by inhibitors). We used this property of the system to investigate two questions: First, what amino acid(s) contribute to species difference in the potency of SphK1 inhibitors, specifically the lower potency of some SphK1 inhibitors at mouse (vs. human) SphK1? Second, what is the minimum size of a functional SphK2 enzyme, specifically, could we generate a functional SphK2 (72 kDa) that is the same size as SphK1 (48 kDa)? The answer to the latter question informs strategies to express recombinant SphK2 for crystallization studies.
We have observed that while SLC5111312 is equipotent at human and rat SphK1 and SphK2, it is a 20-fold SphK2 selective inhibitor in the mouse due to decreased potency at mouse SphK1 [4]. Human and rodent SphK1 amino acid sequences are about 80% identical while mouse and rat SphK1 are 90% identical. Alignment of the human, rat and mouse SphK1 amino acid sequences revealed that position 277 is leucine in mouse but methionine in human and rat. The crystal structures of human SphK1 [21,22] indicate that this amino acid contributes to the sphingosine binding site in the enzyme (SLC5111312 is competitive with sphingosine). To test the hypothesis that Leu277 is responsible for lower potency of SLC5111312 at mouse SphK1, we obtained the mouse SphK1 L277M mutant, determined that its expression was, as expected, growth inhibitory for the CYA169 and KYA1 strains, and determined the potency of two inhibitors at the mutant and WT mouse enzymes. As depicted in Fig 6A, VPC96091, which is an SphK1 inhibitor that does not discriminate between the mouse and human orthologs, is equipotent at wild type and mutant (L277M) mouse SphK1 while SLC5111312 is distinctly more potent at the mutant mouse SphK1 (Fig 6B). The EC 50 values for SLC5111312 were similar for the mutant mouse enzyme and the human enzyme (see Fig 3B).
Human SphK2 is considerably larger than human SphK1 due to an extended (about 130aa) N-terminus and a centrally located 135aa segment. Because both enzymes catalyze the same reactions with similar kinetic constants [23], we hypothesized that deletion of much of the additional amino acid residues in SphK2 would not eliminate enzyme activity and that the smaller SphK2 mutants, if enzymatically active, would be rescued by SphK2 inhibitors. To determine the minimal size of functional human SphK2, we modified the encoding DNA to generate a set of mutants lacking portions of the non-SphK1 overlapping amino acids of human SphK2. The deleted regions are illustrated in Fig 7A. The expression of each deletion mutant was confirmed by western blotting (Fig 7B). Each deletion mutant was tested in the yeast assay for activity (inhibition of growth on galactose media) and, if active, for rescue by the SphK2-selective inhibitor, SLM6031434. The concentration activity curves from the rescue experiment are presented as Fig 8, and the results of the deletion study are summarized in Table 2. Yeast-based sphingolipid kinase assay The replacement of 5 amino acid residues in the course of generating SK2_MOD (see Methods section) from wild type human SphK2 did not impact the assay results. Nearly all the deletion mutants did not grow on galactose (suggesting functional enzyme). All mutants except 15-1 and 33-1 were rescued to some extent by the SphK2 inhibitor, SLM6031434, albeit in some cases with diminished potency. For example, in mutant 11-1, 191 aa were deleted (125 aa from the N-terminus, 66 aa from the central segment) and the enzyme was functional and fully rescued by SLM6031434 with about a 3-fold loss of potency. Deletion of the 44 aa Cterminal region (437-481) of the central segment had a particularly severe, negative effect on Yeast-based sphingolipid kinase assay the potency of the inhibitor (e.g. mutants 26-1, 29-1 and 15-1). An observation that we do not understand is the consistently high background of mutant species 20-1 (N-terminal deletion) on galactose liquid media.

Discussion
Saccharomyces cerevisiae provides a convenient platform for assessing sphingolipid kinase inhibitors. As predicted by previous studies, expression of sphingosine kinases in mutant strains incapable of metabolizing phospho-LCBs results in growth inhibition, and SphK inhibitors restore growth in concert with reducing phospho-LCB levels. We document herein that ceramide kinases are toxic for a standard laboratory strain of yeast (JS1256) and although we presume this toxicity correlates with the accumulation of phospho-dihydroceramide species, this remains to be proven. The assay is particularly useful for screening, but it can also be used in structure-activity profiling of new chemical entities and for conveniently analyzing mutant sphingolipid kinases.
The yeast-based assay is inexpensive and it requires neither specialized equipment nor radioactive material. Although not rapid (24-48 hours), we have found the assay to be robust, indeed, it yielded reliable results in the hands of four undergraduate research assistants. We were unable to reproduce one minor aspect of the Kashem et al. [7] study. That is, we could not achieve sufficient growth of the quadruple null (CBY169) strain seed cultures in media containing raffinose as the fermentable carbon source. We investigated extending the incubation time of the raffinose media seed cultures as well as growing overnight seed cultures in glucose media and switching to raffinose media for two hours before starting the assay. Ultimately, we settled on the expedient strategy of growing overnight seed cultures in glucose media and simply diluting (1:100) these cultures into galactose media to begin our assay. This maneuver probably is responsible for the high, and somewhat variable background (A 600 0.05-0.5) in the assay, but there remains a 10-fold differential between minimal and maximal (A 600 Yeast-based sphingolipid kinase assay 4-6) responses in the tube assay. Further, we did find consistently that both mouse SphK1 and mouse SphK2 grew slower in galactose media than their human orthologs, which necessitated a 40-48 hour rather than a 24 hour assay time but did not otherwise negatively influence the   [7]. The application of the assay suggested initially was to screen chemical libraries for inhibitors of human SphK1 [7]. Our studies suggest that screening for inhibitors of other sphingolipid kinase would also be feasible with this assay-indeed, we have done so with two trypanosomatid sphingosine kinases and human CerK. Screening requires only that compounds accumulate in yeast and not be overtly cytotoxic-success is not strongly dependent on a correlation of inhibitor affinities and the EC 50 values determined with the assay. Kashem et al. [7] suggest that a failure of cytotoxic compounds to be detected is a positive feature of the assay. We agree, but only to the extent that cytotoxicity in yeast predicts cytotoxicity in mammalian cells rather than reflecting some peculiarity of yeast biology, which would be an impediment rather than an advantage.
In contrast to screening chemical libraries, the usefulness of this assay in assessing new chemical entities from a sphingolipid kinase inhibitor medchem campaign depends strongly on the facility of the yeast assay in reporting rank order potencies that reflect the rank order potencies based on inhibitor affinities. In this regard, we are encouraged by our results with one chemical scaffold (1-guanidino-2-phenyloxadiazolylpyrrolidines, e.g. SLM6031434) but we were concerned about the (R)-prolinol scaffold (1-benzyl-2-methanolpyrrolidines, e.g. PF-543) because of the large discrepancy between EC 50 and K I values for PF-543. Subsequent testing of PF-543 analog compounds is required to learn whether the discrepancy is peculiar to PF-543 rather than a general feature the (R)-prolinol chemical scaffold.
The salient limitations of the yeast assay are those of any cell-based assay. Namely, the assay reports EC 50 values rather than K m , V max or k cat values of inhibitors, test compounds must not be markedly cytotoxic at the concentrations tested and compounds must accumulate to some extent in the yeast. Cytotoxicity is readily discerned by testing compounds for an effect on growth of strains without lipid kinase expression. For example, we found that an early generation dual SphK inhibitor, SKI-II, is toxic for yeast strain CBY169 at concentrations of > 3 μM in our assay. Further, we found that compounds such as SLM6031434, SLC5111312 and SLP7111228, which share a pedant 1-guanidino-2-phenyloxadiazolylpyrrolidine group, are likewise toxic at concentrations > 2-3 μM. In the former case, full growth curves cannot be generated and thus an EC 50 value for SKI-II cannot be determined with this assay. In contrast, as documented in Figs 3 and 8, the higher potency guanidino compounds do yield full curves, and thus EC 50 values are obtained.
Accumulation of a test compound in S. cerevisiae can be problematic. If lack of accumulation is due to active extrusion (rather than failure to penetrate and/or metabolism), elimination of candidate transporters, e.g. PDR5p, sometimes provides relief. For example, the human SphK1 inhibitor, PF-543, did not rescue growth of human SphK1-expressing yeast unless the PDR5 gene was deleted (see Fig 3C). In contrast, the potency of the SphK2 inhibitor, SLM6031434, was not different in the presence (CBY169 strain) or absence (KYA1) of PDR5 (Fig 3C), but other 1-guanidino-2-phenyloxadiazolylpyrrolidine compounds were substantially more potent with the KYA1 strain. Since new chemical entities that might be extruded by PDR5p are not recognizable a priori, prudence dictates that library screening and assessment of new chemical entities be conducted with PDR5Δ strains, e.g. KYA1. Our failure to detect activity of ABC294640 at human or mouse SphK2 occurred with both the CBY169 and KYA1 strains, thus we do not know whether this SphK2 inhibitor compound is metabolized rapidly by yeast, fails to penetrate the cell or is extruded by another transporter.
Another limitation is the toxicity of DMSO (dimethylsulfoxide) for Saccharomyces cerevisiae at concentrations > 2% (v/v) in galactose SC media. This toxicity could be nettlesome in screening chemical libraries, which are often arrayed in this solvent. In addition, some exogenous lipid kinases might not be expressed in yeast due to proteolysis or their forced expression might be toxic independent of enzyme activity. Protein expression is readily determined using antisera directed against the N-terminal FLAG epitope (see Fig 7B) and non-enzyme related toxicity can be assessed with catalytically inactive mutant enzymes, but either scenario obviates the assay as a tool to study enzyme inhibitors. We have yet to encounter either of these limitations in our experience to date, which includes the sphingolipid lipid kinases described herein as well as two trypanosomatid sphingosine kinases, pig SphK1 and pig SphK2.
Finally, the usefulness of the yeast platform in interrogating sphingosine and ceramide kinases encouraged us to consider additional applications. One of these is to make advantage of the toxicity of these enzymes to assess mutated forms. We are particularly interested in two questions. Specifically, can a single amino acid change 'humanize' mouse SphK1 in terms of inhibitor affinity? And, what is the minimum size of functional SphK2? The former question is important because numerous disease models are available only in the mouse, but there are few potent mouse SphK1 inhibitors. Our results suggest that a single amino change can humanize mouse Sphk1 for at least one inhibitor and provides a route for rapidly testing other inhibitors. We used the yeast assay to document also that a 'mini' SphK2, which approaches the size of SphK1, is enzymatically competent. The SphK2 deletion series that we describe could prove useful in low resolution mapping of monoclonal antibody epitopes as well as guide expression in E. coli for structural studies.