Effects of six common dietary nutrients on murine intestinal organoid growth
Fig 5
Chlorogenic acid treated organoid growth patterns.
The surface areas of three organoids were measured for each concentration in each day. (A) 5X magnification of organoids given chlorogenic acid. Day 9 –day 14 show organoids with added dietary constituent. Scale bar: 500 μm. (B) 10X magnification. Day 9 –day 14 show organoids with added dietary constituent. Scale bar: 200 μm. (C) Surface area of organoids. (D) Surface area expansion rate of organoids. There was a significant change in growth on Day 9 for 300 μg/mL organoids. However, this change was not observed after Day 9 in any of the concentrations. Significance values were calculated using unpaired, two-tailed Student’s t-test with n = 3. *p<0.05.