Molecular and functional analysis of anchorage independent, treatment-evasive neuroblastoma tumorspheres with enhanced malignant properties: A possible explanation for radio-therapy resistance
Fig 8
Long-term sutent treatment in AI tumorspheres inhibits tumorigenic protein expression and tumorsphere self-renewal.
AI tumorspheres were treated with 0.2 μM sutent for 48 h and then lysed for Western blot analysis. A) Western blot images of representative experiments illustrating the reduced expression of PDGFRβ, MYCN, SOX2 and Survivin after 48 h sutent treatment. B) Densitometric analysis of multiple Western blot experiments showing statistically significant reduction in the protein expression of the above mentioned proteins. C) Micro-images of tumorsphere self-renewal capacity over 15-days, inhibited with 0.2 μM sutent treatment. * indicates a P value < 0.05. Results represent the mean ± SEM of multiple experiments.