Using thermal scanning assays to test protein-protein interactions of inner-ear cadherins
Fig 1
Heterotetrameric tip link made of CDH23 bound to PCDH15.
(A) The tip link is formed by a CDH23 parallel dimer interacting tip-to-tip with a PCDH15 parallel dimer [39]. These proteins feature 27 and 11 extracellular cadherin (EC) repeats, respectively. (B) Ribbon diagram of mouse cdh23 (blue) bound to pcdh15 (magenta) with Ca2+ ions as green spheres (PDB ID: 4APX). Sites of deafness-causing mutations R113 and I108 in PCDH15 are shown in stick representation and circled. (C & D) Detail of I108 (C) and R113 (D) with surrounding residues in the cdh23 and pcdh15 interface.