Dynamical system modeling to simulate donor T cell response to whole exome sequencing-derived recipient peptides: Understanding randomness in alloreactivity incidence following stem cell transplantation
Fig 1
TRaCS, computational algorithm to determine tissue specific alloreactive putative peptide minor histocompatibility antigens (pmHA).
Whole exome sequencing of cryopreserved donor and recipient DNA was performed, with an average coverage of 90x. The variable n refers to the number of nsSNPGVH and m to pmHA. The variable m along with protein tissue expression level was then analyzed in MATLAB to determine T cell responses.